This study examined for the first time the effect of low-energy ultrasound (US), used alone or in combination with methyl jasmonate (MeJA), on viniferin production in cell cultures of Vitis vinifera L. cv Alphonse Lavallée. Cell suspensions were exposed for 2 min to US (power 30, 60, and 90 mW cm−3). The highest viniferin production was obtained at 30 mW cm−3. When sonication was performed twice, the effect on viniferin production was negligible, whereas triple sonication slightly increased production. US treatment at 30 mW cm−3 for 5 min decreased viniferin production and induced cellular death. The combined use of MeJA and US (2 min) increased the production of δ-viniferin, the dominant stilbene, more than each elicitor used alone. These results suggest that low-energy US, alone and in combination with MeJA, can act as a physical elicitor to stimulate viniferin production in V. vinifera cell cultures
Enhancement of Viniferin Production in Vitis vinifera L. cv. Alphonse Lavallée Cell Suspensions by Low-Energy Ultrasound Alone and in Combination with Methyl Jasmonate / Anna Rita Santamaria; Marzia Innocenti; Nadia Mulinacci;Fabrizio Melani;Alessio Valletta; Ilaria Sciandra; Gabriella Pasqua. - In: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. - ISSN 0021-8561. - ELETTRONICO. - 60:(2012), pp. 11135-11142. [10.1021/jf301936u |]
Enhancement of Viniferin Production in Vitis vinifera L. cv. Alphonse Lavallée Cell Suspensions by Low-Energy Ultrasound Alone and in Combination with Methyl Jasmonate
INNOCENTI, MARZIA;MULINACCI, NADIA;MELANI, FABRIZIO;
2012
Abstract
This study examined for the first time the effect of low-energy ultrasound (US), used alone or in combination with methyl jasmonate (MeJA), on viniferin production in cell cultures of Vitis vinifera L. cv Alphonse Lavallée. Cell suspensions were exposed for 2 min to US (power 30, 60, and 90 mW cm−3). The highest viniferin production was obtained at 30 mW cm−3. When sonication was performed twice, the effect on viniferin production was negligible, whereas triple sonication slightly increased production. US treatment at 30 mW cm−3 for 5 min decreased viniferin production and induced cellular death. The combined use of MeJA and US (2 min) increased the production of δ-viniferin, the dominant stilbene, more than each elicitor used alone. These results suggest that low-energy US, alone and in combination with MeJA, can act as a physical elicitor to stimulate viniferin production in V. vinifera cell culturesFile | Dimensione | Formato | |
---|---|---|---|
Vitis_sonication_JAFC(2012).pdf
Accesso chiuso
Tipologia:
Altro
Licenza:
Tutti i diritti riservati
Dimensione
343.19 kB
Formato
Adobe PDF
|
343.19 kB | Adobe PDF | Richiedi una copia |
I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.