In-cell NMR for structural and functional studies of proteins in their native environment

In this PhD thesis, the in-cell NMR approach was applied to study protein folding and maturation processes at atomic level in living cells. This approach, which relies on protein overexpression, was first applied on living bacterial cells, and was subsequently extended to cultured human cells. Observing the proteins in human cells allowed understanding how the correct cellular environment influences protein folding and redox state, how it controls the availability and binding of the metal cofactors, and how it mediates the effect of specific interacting partners in ways which are yet to be modelled in vitro.