β-adrenergic receptors (β-ARs) are G protein-coupled receptors that activate signal transduction pathways involved in angiogenesis, resulting in enhanced tumor vascularization and more aggressive growth. In this study, we evaluated the expression of β-ARs in a population of 12 children affected by malignant primary brain tumors. We found a significant expression of β1- and β2-ARs in all 12 samples as well as the 3 cell lines tested (U87MG, T98G and DAOY). The mean absolute β1-AR mRNA level standardized to GAPDH was 5.81 (range, -7.91 to 11.29) for brain tumors and 8.59 (range, 6.046 to 12.59) for cell lines (U87MG, DAOY and T98G), respectively. The mean absolute β2-AR mRNA level was 4.74 (range, -9.30 to 8.45) for tumor specimens and 7.64 (range, 5.85 to 8.88) for cell lines. These real-time quantitative (qRT)-PCR expression data were confirmed by immunohistochemical analysis. Our study evaluated the presence of β1- and β2-ARs in malignant pediatric brain tumors and brain tumor cell lines.

Expression of β-adrenergic receptors in pediatric malignant brain tumors / Sardi I;Giunti L;Bresci C;Buccoliero AM;Degl'innocenti D;Cardellicchio S;Baroni G;Castiglione F;Ros MD;Fiorini P;Giglio S;Genitori L;Aricò M;Filippi L. - In: ONCOLOGY LETTERS. - ISSN 1792-1074. - STAMPA. - 5:(2013), pp. 221-225. [10.3892/ol.2012.989]

Expression of β-adrenergic receptors in pediatric malignant brain tumors.

BUCCOLIERO, ANNA MARIA;ROSSI DEGL'INNOCENTI, DUCCIO;CASTIGLIONE, FRANCESCA;GIGLIO, SABRINA RITA;
2013

Abstract

β-adrenergic receptors (β-ARs) are G protein-coupled receptors that activate signal transduction pathways involved in angiogenesis, resulting in enhanced tumor vascularization and more aggressive growth. In this study, we evaluated the expression of β-ARs in a population of 12 children affected by malignant primary brain tumors. We found a significant expression of β1- and β2-ARs in all 12 samples as well as the 3 cell lines tested (U87MG, T98G and DAOY). The mean absolute β1-AR mRNA level standardized to GAPDH was 5.81 (range, -7.91 to 11.29) for brain tumors and 8.59 (range, 6.046 to 12.59) for cell lines (U87MG, DAOY and T98G), respectively. The mean absolute β2-AR mRNA level was 4.74 (range, -9.30 to 8.45) for tumor specimens and 7.64 (range, 5.85 to 8.88) for cell lines. These real-time quantitative (qRT)-PCR expression data were confirmed by immunohistochemical analysis. Our study evaluated the presence of β1- and β2-ARs in malignant pediatric brain tumors and brain tumor cell lines.
2013
5
221
225
Sardi I;Giunti L;Bresci C;Buccoliero AM;Degl'innocenti D;Cardellicchio S;Baroni G;Castiglione F;Ros MD;Fiorini P;Giglio S;Genitori L;Aricò M;Filippi L...espandi
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/795270
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