Flow Synthesis and Biological Studies of an Analgesic Adamantane Derivative That Inhibits P2X7‐Evoked Glutamate Release

We report the biological evaluation of a class of adamantane derivatives, which were achieved via modified telescoped machine-assisted flow procedure. Among the series of compounds tested in this work, 5 demonstrated outstanding analgesic properties. This compound showed that its action was not mediated through direct interaction with opioid and/ or cannabinoid receptors. Moreover, it did not display any significant anti-inflammatory properties. Experiments carried out on rat cerebrocortical purified synaptosomes indicated that 5 inhibits the P2X7-evoked glutamate release, which may contribute to its antinociceptive properties. Nevertheless, further experiments are ongoing to characterize the pharmacological properties and mechanism of action of this molecule. KEYWORDS: Adamantane derivatives, 2-aminoadamantane-2-carboxylic acid, analgesic agents, flow chemistry, purinergic receptors