Staphylococcus aureus is one of the major human pathogens at both nosocomial and community level. Resistance to antiseptics and disinfectants based on quaternary ammonium compounds (QACs) is widespread among staphylococci. Resistance to QACs is often associated to the presence of plasmid encoded efflux pumps or to up-regulation of the chromosomal NorA pump. Four different qac genes, including qacA, qacB, smr (qacC) and qacG are found in clinical isolates. We applied the Phenotype Microarray (PM) high-throughput technology (which allows to test the chemical sensitivity patterns, analyzing metabolic activity, in presence of 240 different antimicrobial compounds, each one at 4 concentrations) to compare the metabolic activity of 15 strains of S. aureus bearing qacA, qacB, qacC and qacG determinants with 5 qac-negative strain in order to deepen the knowledge on responses associated to different qac pumps and to identify new potential efflux substrates.
Novel antimicrobial resistance phenotypes associated to qac and norA genotypes in Staphylococcus aureus / E. Marchi; L. Furi; S. Arioli; I. Morrissey; D. Mora; L. Giovannetti; M. R. Oggioni; C. Viti.. - STAMPA. - (2013), pp. p-p. (Intervento presentato al convegno 7th Conference on Functional Genomics of Gram-positive Microorganisms tenutosi a Montecatini Terme nel 23-27 giugno 2013).
Novel antimicrobial resistance phenotypes associated to qac and norA genotypes in Staphylococcus aureus
GIOVANNETTI, LUCIANA;VITI, CARLO
2013
Abstract
Staphylococcus aureus is one of the major human pathogens at both nosocomial and community level. Resistance to antiseptics and disinfectants based on quaternary ammonium compounds (QACs) is widespread among staphylococci. Resistance to QACs is often associated to the presence of plasmid encoded efflux pumps or to up-regulation of the chromosomal NorA pump. Four different qac genes, including qacA, qacB, smr (qacC) and qacG are found in clinical isolates. We applied the Phenotype Microarray (PM) high-throughput technology (which allows to test the chemical sensitivity patterns, analyzing metabolic activity, in presence of 240 different antimicrobial compounds, each one at 4 concentrations) to compare the metabolic activity of 15 strains of S. aureus bearing qacA, qacB, qacC and qacG determinants with 5 qac-negative strain in order to deepen the knowledge on responses associated to different qac pumps and to identify new potential efflux substrates.
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