In vitro strategies to obtain different T helper cell subsets from naïve precursors are usually characterized by anti-CD3 plus anti-CD28 monoclonal antibody stimulation of naïve CD4+ T cells (derived from both peripheral blood or umbilical cord blood) in the presence of specifi c polarizing cytokines. In this chapter, we describe detailed protocols to obtain in vitro differentiation of human naïve CD4+ T cells into Th1, Th2, or Th17 cells.
Strategies for T Helper Cell Subset Differentiation from Naïve Precursors / Annunziato, Francesco; Maggi, Laura. - STAMPA. - (2017), pp. 127-137. [10.1007/978-1-4939-6548-9_11]
Strategies for T Helper Cell Subset Differentiation from Naïve Precursors
ANNUNZIATO, FRANCESCO;MAGGI, LAURA
2017
Abstract
In vitro strategies to obtain different T helper cell subsets from naïve precursors are usually characterized by anti-CD3 plus anti-CD28 monoclonal antibody stimulation of naïve CD4+ T cells (derived from both peripheral blood or umbilical cord blood) in the presence of specifi c polarizing cytokines. In this chapter, we describe detailed protocols to obtain in vitro differentiation of human naïve CD4+ T cells into Th1, Th2, or Th17 cells.
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