Studies of the effects of phosphate (Pi) on force generation in skinned muscle preparartion have been usually restricted to Pi concentrations greater than 150-200 uM because of the inability to buffer the diffusive accumulation of Pi liberated within the fibres (50-100 um wide) by the actomyosin ATPase.Single myofibrils or thin bundles of few myofibrils (1-3 um wide) isolated from rabbit psoas have been used here to overcome this limitation.
The effects of phosphate on the contraction of single skeletal myofibrils / N. Piroddi, P. Caini, F. Colomo, S. Nencini, C. Poggesi, C. Tesi. - In: PFLÜGERS ARCHIV. - ISSN 0031-6768. - STAMPA. - 438:(1999), pp. R11-R11.
The effects of phosphate on the contraction of single skeletal myofibrils
N. Piroddi;P. Caini;F. Colomo;NENCINI, SIMONE;C. Poggesi;C. Tesi
1999
Abstract
Studies of the effects of phosphate (Pi) on force generation in skinned muscle preparartion have been usually restricted to Pi concentrations greater than 150-200 uM because of the inability to buffer the diffusive accumulation of Pi liberated within the fibres (50-100 um wide) by the actomyosin ATPase.Single myofibrils or thin bundles of few myofibrils (1-3 um wide) isolated from rabbit psoas have been used here to overcome this limitation.
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