During my PhD, I designed and developed a custom-made multimodal microscope for combining Fluorescence Lifetime Imaging Microscopy (FLIM), Second Harmonic Generation (SHG), Two-Photon Excited Fluorescence (TPEF) and Raman microscopy for studying both tumour and non-tumour diseases. Then, I conducted three research studies based on the biomedical applications of these optical techniques. I used two-photon microscopy for examining ex vivo human carotids affected by atherosclerosis, and Raman spectroscopy for discriminating three in vitro melanoma cell lines. Moreover, I combined TPEF and Raman microscopy for studying two ex vivo “bulk” tissue types: human bladder affected by urothelial carcinoma (UC) and atherosclerotic aorta obtained from an animal model. Both two-photon and Raman microscopy proved to be valuable tools for analysing biological samples.
Multimodal imaging for tissue diagnostics by combined two-photon and Raman microscopy / Enrico Baria. - (2018).
Multimodal imaging for tissue diagnostics by combined two-photon and Raman microscopy
Enrico Baria
2018
Abstract
During my PhD, I designed and developed a custom-made multimodal microscope for combining Fluorescence Lifetime Imaging Microscopy (FLIM), Second Harmonic Generation (SHG), Two-Photon Excited Fluorescence (TPEF) and Raman microscopy for studying both tumour and non-tumour diseases. Then, I conducted three research studies based on the biomedical applications of these optical techniques. I used two-photon microscopy for examining ex vivo human carotids affected by atherosclerosis, and Raman spectroscopy for discriminating three in vitro melanoma cell lines. Moreover, I combined TPEF and Raman microscopy for studying two ex vivo “bulk” tissue types: human bladder affected by urothelial carcinoma (UC) and atherosclerotic aorta obtained from an animal model. Both two-photon and Raman microscopy proved to be valuable tools for analysing biological samples.File | Dimensione | Formato | |
---|---|---|---|
PhD thesis.pdf
Open Access dal 10/03/2019
Descrizione: Tesi di dottorato
Tipologia:
Tesi di dottorato
Licenza:
Open Access
Dimensione
5.68 MB
Formato
Adobe PDF
|
5.68 MB | Adobe PDF |
I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.