Functional foods offer, in addition to their high nutritional value, a health advantage due to the beneficial activities of their natural bioactive components. Several microalgae have been proposed as substrate for functional foods. In this study we evaluated the anti-inflammatory effects of the methanolic extract of Tisochrysis lutea F&M-M36 and the aqueous extract of Arthrospira platensis F&M-C256 by determining their inhibitory effects on pro-inflammatory mediators in lipopolysaccharide (LPS)-stimulated murine macrophage RAW cells. Their effects were compared to those of pure fucoxanthin (FX) and phycocyanin (PC). The expression of COX2, iNOs and heme oxygenase-1 (HO-1) genes was measured by RT-PCR in the cellular lysate and PGE2 concentration was quantified in the culture medium by using a competitive enzyme immunoassay. The methanolic extract from T. lutea F&M-M36 was able to reduce the LPS-stimulated production of PGE2, dose-dependently, exhibiting a higher effect compared to that of FX at a concentration comparable to that present in the microalgal biomass. On the contrary, only the water-soluble phycobiliprotein PC, modulated significantly the expression of the pro-inflammatory genes COX2 and iNOs. However, both extracts and single compounds up-regulated the expression of HO-1 in the presence of LPS, suggesting the involvement of the Nrf2/ARE signaling pathway that plays an important role in inhibiting the production of pro-inflammatory cytokines. In conclusion, these preliminary data indicate that T. lutea F&M-M36 and A. platensis F&M-C256 extracts inhibit inflammatory responses via the up-regulation of Nrf2/HO-1 pathway. These two microalgae have potential for the control of inflammatory chronic diseases.

Antinflammatory effects of Tisochrysis lutea F&M-M36 and Arthrospira platensis F&M-C256 extracts / Bigagli E., Cinci L., Niccolai A., Tredici M.R., Biondi N., Rodolfi L., D’Ambrosio M., Luceri C. - ELETTRONICO. - (2018), pp. 270-271. ((Intervento presentato al convegno 5th EABA and EC Contractors' Conference and the 12th International Algae Congress tenutosi a Amsterdam, The Netherlands nel 04/12/2018 - 06/12/2018.

Antinflammatory effects of Tisochrysis lutea F&M-M36 and Arthrospira platensis F&M-C256 extracts

Bigagli E.
;
Cinci L.;Niccolai A.;Tredici M. R.;Biondi N.;Rodolfi L.;Luceri C
2018

Abstract

Functional foods offer, in addition to their high nutritional value, a health advantage due to the beneficial activities of their natural bioactive components. Several microalgae have been proposed as substrate for functional foods. In this study we evaluated the anti-inflammatory effects of the methanolic extract of Tisochrysis lutea F&M-M36 and the aqueous extract of Arthrospira platensis F&M-C256 by determining their inhibitory effects on pro-inflammatory mediators in lipopolysaccharide (LPS)-stimulated murine macrophage RAW cells. Their effects were compared to those of pure fucoxanthin (FX) and phycocyanin (PC). The expression of COX2, iNOs and heme oxygenase-1 (HO-1) genes was measured by RT-PCR in the cellular lysate and PGE2 concentration was quantified in the culture medium by using a competitive enzyme immunoassay. The methanolic extract from T. lutea F&M-M36 was able to reduce the LPS-stimulated production of PGE2, dose-dependently, exhibiting a higher effect compared to that of FX at a concentration comparable to that present in the microalgal biomass. On the contrary, only the water-soluble phycobiliprotein PC, modulated significantly the expression of the pro-inflammatory genes COX2 and iNOs. However, both extracts and single compounds up-regulated the expression of HO-1 in the presence of LPS, suggesting the involvement of the Nrf2/ARE signaling pathway that plays an important role in inhibiting the production of pro-inflammatory cytokines. In conclusion, these preliminary data indicate that T. lutea F&M-M36 and A. platensis F&M-C256 extracts inhibit inflammatory responses via the up-regulation of Nrf2/HO-1 pathway. These two microalgae have potential for the control of inflammatory chronic diseases.
Abstract book 5th AlgaEurope Conference
5th EABA and EC Contractors' Conference and the 12th International Algae Congress
Amsterdam, The Netherlands
04/12/2018 - 06/12/2018
Bigagli E., Cinci L., Niccolai A., Tredici M.R., Biondi N., Rodolfi L., D’Ambrosio M., Luceri C
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2158/1145584
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