Recent advances in the preparation and characterization of metal core-based nanoparticles have opened the way to their exploration as carriers for carbohydrate-based vaccines. Here we describe the protocols for the preparation and characterization of water dispersible gold nanoparticles (1-3 nm gold diameter) as carriers for carbohydrate antigens. We mainly refer to the protocols we used for the preparation of gold glyconanoparticles as carrier for an S. pneumoniae carbohydrate-antigen. The high number of ligands at the gold nanoparticles surface and the easiness of their one-pot preparation make these biocompatible nanomaterials an attractive tool for glyco-scientists.
Gold Nanoparticles as Carriers for Synthetic Glycoconjugate Vaccines / Chiodo, Fabrizio; Marradi, Marco. - ELETTRONICO. - (2015), pp. 159-171. [10.1007/978-1-4939-2874-3_10]
Gold Nanoparticles as Carriers for Synthetic Glycoconjugate Vaccines
Marradi, Marco
2015
Abstract
Recent advances in the preparation and characterization of metal core-based nanoparticles have opened the way to their exploration as carriers for carbohydrate-based vaccines. Here we describe the protocols for the preparation and characterization of water dispersible gold nanoparticles (1-3 nm gold diameter) as carriers for carbohydrate antigens. We mainly refer to the protocols we used for the preparation of gold glyconanoparticles as carrier for an S. pneumoniae carbohydrate-antigen. The high number of ligands at the gold nanoparticles surface and the easiness of their one-pot preparation make these biocompatible nanomaterials an attractive tool for glyco-scientists.
I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
