We propose two different approaches involving the use of quantitative real-time PCR for the detection or analysis of circulating tumor cells. In one case cells are indirectly identified through the expression of a marker mRNA, while in the other one cells are enriched by size prior to be submitted to mutational analysis for a specific target. Both methods have been successfully applied to the study of circulating melanoma cells.
Detection and Characterization of Circulating Tumor Cells by Quantitative Real-Time PCR / Salvianti F.; Costanza F.; Sonnati G.; Pinzani P.. - ELETTRONICO. - (2020), pp. 139-151. [10.1007/978-1-4939-9833-3_11]
Detection and Characterization of Circulating Tumor Cells by Quantitative Real-Time PCR
Salvianti F.;Costanza F.;Sonnati G.;Pinzani P.
2020
Abstract
We propose two different approaches involving the use of quantitative real-time PCR for the detection or analysis of circulating tumor cells. In one case cells are indirectly identified through the expression of a marker mRNA, while in the other one cells are enriched by size prior to be submitted to mutational analysis for a specific target. Both methods have been successfully applied to the study of circulating melanoma cells.
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