Algae and bacteria establish complex relationships reciprocally influencing their growth. Associated bacterial communities alter microalgae metabolism and physiology, affecting biomass yield and quality. In this study, 203 bacterial isolates (33 different genera), recovered from Tetraselmis suecica F&M-M33 cultures, were identified and phenotypically characterized in relation to the microalgal growth-promoting (MGP) features of indol-3-acetic acid (IAA) and siderophore production. IAA production was observed in 30 isolates belonging to 10 genera, whilst siderophore production was observed in 12 isolates belonging to nine genera, indicating that bacteria which are phylogenetically different within the phycosphere community may contribute in similar ways to boosting algal growth. Moreover, strains belonging to a single genus (e.g. Bacillus, Muricauda, Labrenzia and Amorphus) showed different MGP features, but none of these isolates were capable of producing both growth-promoting factors. Twenty-two selected strains were further tested in co-culture assays to evaluate their effect on T. suecica F&M-M33 growth; four induced an increase in microalgal productivity. The exo-metabolome of T. suecica F&M-M33 cultures, either axenically or when co-cultured with either Vitellibacter strain AAD2 or Sphingopyxis flavimaris strain AG5, was determined using a non-targeted metabolomics approach using an Ultra High Performance Liquid Chromatography (UHPLC) system. This allowed for the detection of 133 entities (small chemical molecules, e.g. metabolites), of which 84 were identified. Most of the entities were common in all three cultures, showing that the T. suecica F&M-M33 phycosphere was rich in small peptides and organic acids, and a high number of terpenes were also observed. Seventeen entities were culture-specific, suggesting that they were directly related to microalgae–bacteria interactions.

Tetraselmis suecica F&M-M33 phycosphere: associated bacteria and exo-metabolome characterisation / Piampiano, Elisa; Pini, Francesco; Biondi, Natascia; Garcia, Carlos J.; Decorosi, Francesca; Tomàs-Barberàn, Francisco A.; Giovannetti, Luciana; Viti, Carlo. - In: EUROPEAN JOURNAL OF PHYCOLOGY. - ISSN 0967-0262. - STAMPA. - 56:(2021), pp. 61-71. [10.1080/09670262.2020.1765024]

Tetraselmis suecica F&M-M33 phycosphere: associated bacteria and exo-metabolome characterisation

Piampiano, Elisa;Pini, Francesco;Biondi, Natascia;Decorosi, Francesca;Giovannetti, Luciana;Viti, Carlo
2021

Abstract

Algae and bacteria establish complex relationships reciprocally influencing their growth. Associated bacterial communities alter microalgae metabolism and physiology, affecting biomass yield and quality. In this study, 203 bacterial isolates (33 different genera), recovered from Tetraselmis suecica F&M-M33 cultures, were identified and phenotypically characterized in relation to the microalgal growth-promoting (MGP) features of indol-3-acetic acid (IAA) and siderophore production. IAA production was observed in 30 isolates belonging to 10 genera, whilst siderophore production was observed in 12 isolates belonging to nine genera, indicating that bacteria which are phylogenetically different within the phycosphere community may contribute in similar ways to boosting algal growth. Moreover, strains belonging to a single genus (e.g. Bacillus, Muricauda, Labrenzia and Amorphus) showed different MGP features, but none of these isolates were capable of producing both growth-promoting factors. Twenty-two selected strains were further tested in co-culture assays to evaluate their effect on T. suecica F&M-M33 growth; four induced an increase in microalgal productivity. The exo-metabolome of T. suecica F&M-M33 cultures, either axenically or when co-cultured with either Vitellibacter strain AAD2 or Sphingopyxis flavimaris strain AG5, was determined using a non-targeted metabolomics approach using an Ultra High Performance Liquid Chromatography (UHPLC) system. This allowed for the detection of 133 entities (small chemical molecules, e.g. metabolites), of which 84 were identified. Most of the entities were common in all three cultures, showing that the T. suecica F&M-M33 phycosphere was rich in small peptides and organic acids, and a high number of terpenes were also observed. Seventeen entities were culture-specific, suggesting that they were directly related to microalgae–bacteria interactions.
2021
56
61
71
Goal 14: Life below water
Piampiano, Elisa; Pini, Francesco; Biondi, Natascia; Garcia, Carlos J.; Decorosi, Francesca; Tomàs-Barberàn, Francisco A.; Giovannetti, Luciana; Viti,...espandi
File in questo prodotto:
File Dimensione Formato  
Tetraselmis suecica F M M33 phycosphere associated bacteria and exo metabolome characterisation.pdf

Accesso chiuso

Tipologia: Pdf editoriale (Version of record)
Licenza: Tutti i diritti riservati
Dimensione 1.53 MB
Formato Adobe PDF
1.53 MB Adobe PDF   Richiedi una copia

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1197597
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 6
  • ???jsp.display-item.citation.isi??? 8
social impact