The interaction between neurons and glia is pivotal for the development of chronic opioid tolerance. One of the most important mechanisms of cell-to-cell interaction is the Notch signaling pathway. In this chapter we propose a double-immunofluorescence method to observe and quantify the colocalization of Notch-1 and mu-opioid receptor (MOR-1), using both neuronal and astrocyte markers.

Fluorescence colocalization analysis of cellular distribution of mor-1 / Borgonetti V.; Galeotti N.. - STAMPA. - (2021), pp. 27-34. [10.1007/978-1-0716-0884-5_3]

Fluorescence colocalization analysis of cellular distribution of mor-1

Borgonetti V.;Galeotti N.
2021

Abstract

The interaction between neurons and glia is pivotal for the development of chronic opioid tolerance. One of the most important mechanisms of cell-to-cell interaction is the Notch signaling pathway. In this chapter we propose a double-immunofluorescence method to observe and quantify the colocalization of Notch-1 and mu-opioid receptor (MOR-1), using both neuronal and astrocyte markers.
978-1-0716-0883-8
978-1-0716-0884-5
Methods in Molecular Biology
27
34
Goal 3: Good health and well-being
Borgonetti V.; Galeotti N.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2158/1208550
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 2
  • ???jsp.display-item.citation.isi??? ND
social impact