The interaction between neurons and glia is pivotal for the development of chronic opioid tolerance. One of the most important mechanisms of cell-to-cell interaction is the Notch signaling pathway. In this chapter we propose a double-immunofluorescence method to observe and quantify the colocalization of Notch-1 and mu-opioid receptor (MOR-1), using both neuronal and astrocyte markers.
Fluorescence colocalization analysis of cellular distribution of mor-1 / Borgonetti V.; Galeotti N.. - STAMPA. - (2021), pp. 27-34. [10.1007/978-1-0716-0884-5_3]
Fluorescence colocalization analysis of cellular distribution of mor-1
Borgonetti V.;Galeotti N.
2021
Abstract
The interaction between neurons and glia is pivotal for the development of chronic opioid tolerance. One of the most important mechanisms of cell-to-cell interaction is the Notch signaling pathway. In this chapter we propose a double-immunofluorescence method to observe and quantify the colocalization of Notch-1 and mu-opioid receptor (MOR-1), using both neuronal and astrocyte markers.
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