Vaccination has had a formidable positive effect on the quality of public health, essentially eliminating most of the life-threatening infectious diseases and saving millions of lives, in particular infants and young children. In this regard, glycoconjugate vaccines, based on the covalent linking to immunogenic proteins of glycan structures uniquely exposed on the surface of pathogens as well as malignant cells, represent one of the greatest success stories in the biomedical sciences. Glycoconjugate vaccines indeed exert a satisfying immunological activity even among persons in high-risk groups, and several conjugate versions of polysaccharide vaccines are now either commercially available or in development. However, the occurrence of still unmet medical needs as well as the unceasing emergence of multidrug resistant strains strongly demand the advent of novel and more efficient glycoconjugate vaccines. In addition, to timely counteract the spread of diseases, these new constructs should benefit from an easier physicochemical characterization in order to streamline their production process and marketing. The present chapter describes the key characteristics of the three essential components of a glycoconjugate, i.e. the saccharide antigen, the linker and the protein carrier. In particular, starting from the state-of-the-art of the currently licensed glycoconjugate vaccines, the major strategies (random vs selective) adopted for the chemical modification of both the carbohydrate antigen and the carrier protein, and their subsequent assembly are dissected and critically discussed.

Conjugation Techniques and Linker Strategies for Carbohydrate-Based Vaccines / Barbara Richichi; Giacomo Biagiotti; Luigi Lay; Giuseppe Stefanetti;. - ELETTRONICO. - (2021), pp. 676-705. [10.1016/B978-0-12-819475-1.00089-4]

Conjugation Techniques and Linker Strategies for Carbohydrate-Based Vaccines

Barbara Richichi;Giacomo Biagiotti;
2021

Abstract

Vaccination has had a formidable positive effect on the quality of public health, essentially eliminating most of the life-threatening infectious diseases and saving millions of lives, in particular infants and young children. In this regard, glycoconjugate vaccines, based on the covalent linking to immunogenic proteins of glycan structures uniquely exposed on the surface of pathogens as well as malignant cells, represent one of the greatest success stories in the biomedical sciences. Glycoconjugate vaccines indeed exert a satisfying immunological activity even among persons in high-risk groups, and several conjugate versions of polysaccharide vaccines are now either commercially available or in development. However, the occurrence of still unmet medical needs as well as the unceasing emergence of multidrug resistant strains strongly demand the advent of novel and more efficient glycoconjugate vaccines. In addition, to timely counteract the spread of diseases, these new constructs should benefit from an easier physicochemical characterization in order to streamline their production process and marketing. The present chapter describes the key characteristics of the three essential components of a glycoconjugate, i.e. the saccharide antigen, the linker and the protein carrier. In particular, starting from the state-of-the-art of the currently licensed glycoconjugate vaccines, the major strategies (random vs selective) adopted for the chemical modification of both the carbohydrate antigen and the carrier protein, and their subsequent assembly are dissected and critically discussed.
9780128222447
Conjugation Techniques and Linker Strategies for Carbohydrate-Based Vaccines
676
705
Barbara Richichi; Giacomo Biagiotti; Luigi Lay; Giuseppe Stefanetti;
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2158/1245096
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