Oleuropein, the major compound found in olive leaves, has been reported to exert numerous pharmacological properties, including anti-inflammatory, anti-diabetic and anti-cancer effects. The purpose of this study was to evaluate, for the first time, the effect of oleuropein-rich leaf extracts (ORLE) in already-developed colon tumours arising in Apc (adenomatous polyposis coli) mutated PIRC rats (F344/NTac-Apcam1137). Here, we were able to investigate in parallel the anti-cancer effect of ORLE, both in vivo and in vitro, and its anti-inflammatory effect on macrophages, representing a critical and abundant population in most solid tumour microenvironment. We found that in vivo ORLE treatment promoted apoptosis and attenuated iNOS activity both in colon tumours as in peritoneal macrophages of PIRC rats. We this confirmed in vitro using primary RAW264.7 cells: ORLE reduced iNOS activity in parallel with COX-2 and pro-inflammatory cytokines, such as IL-1β, IL-6 and TGF-β. These findings suggest that ORLE possess a strong anti-inflammatory activity, which could be crucial for dampening the pro-tumourigenic activity elicited by a chronic inflammatory state generated by either tumour cells or tumour-associated macrophages.

Oleuropein-rich leaf extract as a broad inhibitor of tumour and macrophage iNOS in an apc mutant rat model / Jessica Ruzzolini , Sofia Chioccioli , Noemi Monaco , Silvia Peppicelli , Elena Andreucci , Silvia Urciuoli , Annalisa Romani, Cristina Luceri , Katia Tortora , Lido Calorini , Giovanna Caderni , Chiara Nediani , Francesca Bianchini. - In: ANTIOXIDANTS. - ISSN 2076-3921. - ELETTRONICO. - 10:(2021), pp. 0-0. [10.3390/antiox10101577]

Oleuropein-rich leaf extract as a broad inhibitor of tumour and macrophage iNOS in an apc mutant rat model

Silvia Urciuoli;Annalisa Romani
Membro del Collaboration Group
;
Cristina Luceri
Membro del Collaboration Group
;
Katia Tortora
Investigation
;
Lido Calorini
Conceptualization
;
Giovanna Caderni
Conceptualization
;
Chiara Nediani
Supervision
;
Francesca Bianchini
Writing – Original Draft Preparation
2021

Abstract

Oleuropein, the major compound found in olive leaves, has been reported to exert numerous pharmacological properties, including anti-inflammatory, anti-diabetic and anti-cancer effects. The purpose of this study was to evaluate, for the first time, the effect of oleuropein-rich leaf extracts (ORLE) in already-developed colon tumours arising in Apc (adenomatous polyposis coli) mutated PIRC rats (F344/NTac-Apcam1137). Here, we were able to investigate in parallel the anti-cancer effect of ORLE, both in vivo and in vitro, and its anti-inflammatory effect on macrophages, representing a critical and abundant population in most solid tumour microenvironment. We found that in vivo ORLE treatment promoted apoptosis and attenuated iNOS activity both in colon tumours as in peritoneal macrophages of PIRC rats. We this confirmed in vitro using primary RAW264.7 cells: ORLE reduced iNOS activity in parallel with COX-2 and pro-inflammatory cytokines, such as IL-1β, IL-6 and TGF-β. These findings suggest that ORLE possess a strong anti-inflammatory activity, which could be crucial for dampening the pro-tumourigenic activity elicited by a chronic inflammatory state generated by either tumour cells or tumour-associated macrophages.
2021
10
0
0
Jessica Ruzzolini , Sofia Chioccioli , Noemi Monaco , Silvia Peppicelli , Elena Andreucci , Silvia Urciuoli , Annalisa Romani, Cristina Luceri , Katia Tortora , Lido Calorini , Giovanna Caderni , Chiara Nediani , Francesca Bianchini
File in questo prodotto:
File Dimensione Formato  
OLE e PIRC.pdf

accesso aperto

Tipologia: Pdf editoriale (Version of record)
Licenza: Open Access
Dimensione 2.6 MB
Formato Adobe PDF
2.6 MB Adobe PDF

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1246037
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 15
  • ???jsp.display-item.citation.isi??? 16
social impact