Human T cells represent a heterogeneous population, including cells with different phenotypical and function properties. Despite, in the last years, several technologies were developed to investigate phenotypical properties of T cells at single cell level, in vitro T cell clone’s culture remains the only way to perform functional study on T cells at single cell levels. Here, we describe the method to obtain human T cell clones by limiting dilution in the presence of feeder cells and to maintain them in culture for further investigations.

Human T-cell cloning by limiting dilution / Maggi L.; Capone M.; Mazzoni A.. - STAMPA. - (2021), pp. 165-172. [10.1007/978-1-0716-1311-5_14]

Human T-cell cloning by limiting dilution

Maggi L.
;
Capone M.;Mazzoni A.
2021

Abstract

Human T cells represent a heterogeneous population, including cells with different phenotypical and function properties. Despite, in the last years, several technologies were developed to investigate phenotypical properties of T cells at single cell level, in vitro T cell clone’s culture remains the only way to perform functional study on T cells at single cell levels. Here, we describe the method to obtain human T cell clones by limiting dilution in the presence of feeder cells and to maintain them in culture for further investigations.
2021
978-1-0716-1310-8
978-1-0716-1311-5
Methods in Molecular Biology
165
172
Maggi L.; Capone M.; Mazzoni A.
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1259303
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