Flow cytometric evaluation of phosphorylation status of signal transduction molecules is a useful method to study T-cell signaling pathways. As mutations occurring in TCR complex molecules, common gamma chain family’s cytokines, their receptors or molecules involved in these pathways can lead to severe immune system defects, the study of T-cell signal transduction can be applied to both basic and clinical/translational research areas. In the present chapter, we show two different protocols for the study of T- cell response to an antigen-like stimulus and to IL-2.
Study of signal transduction pathways by phospho-protein evaluation / Capone M.; Mazzoni A.; Maggi L.. - STAMPA. - (2021), pp. 191-200. [10.1007/978-1-0716-1311-5_16]
Study of signal transduction pathways by phospho-protein evaluation
Capone M.;Mazzoni A.;Maggi L.
2021
Abstract
Flow cytometric evaluation of phosphorylation status of signal transduction molecules is a useful method to study T-cell signaling pathways. As mutations occurring in TCR complex molecules, common gamma chain family’s cytokines, their receptors or molecules involved in these pathways can lead to severe immune system defects, the study of T-cell signal transduction can be applied to both basic and clinical/translational research areas. In the present chapter, we show two different protocols for the study of T- cell response to an antigen-like stimulus and to IL-2.I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.