DNA extraction from plant samples is very important for a good performance of diagnostic molecular assays in phytopathology. The variety of matrices (such as leaves, roots, and twigs) requires a differentiated approach to DNA extraction. Here we describe three categories of matrices: (a) symptomatic bark/wood tissue; (b) residues of frass resulting from insect woody trophic activities, portions of the galleries produced in the wood, and tissues surrounding exit holes; and (c) leaves of different plant species. To improve the performances of diagnostic assays, we here describe DNA extraction procedures that have been optimized for each matrix type.

DNA extraction methods to obtain high DNA quality from different plant tissues / Domenico Rizzo, Daniele Da Lio, Linda bartolini, Cristina Franci, Antonio Aronadio, Nicola Luchi, Sara Campigli, Guido Marchi, Elisabetta Rossi. - STAMPA. - (2022), pp. 91-101.

DNA extraction methods to obtain high DNA quality from different plant tissues

Sara Campigli;Guido Marchi;
2022

Abstract

DNA extraction from plant samples is very important for a good performance of diagnostic molecular assays in phytopathology. The variety of matrices (such as leaves, roots, and twigs) requires a differentiated approach to DNA extraction. Here we describe three categories of matrices: (a) symptomatic bark/wood tissue; (b) residues of frass resulting from insect woody trophic activities, portions of the galleries produced in the wood, and tissues surrounding exit holes; and (c) leaves of different plant species. To improve the performances of diagnostic assays, we here describe DNA extraction procedures that have been optimized for each matrix type.
Methods in Molecular Biology
91
101
Domenico Rizzo, Daniele Da Lio, Linda bartolini, Cristina Franci, Antonio Aronadio, Nicola Luchi, Sara Campigli, Guido Marchi, Elisabetta Rossi
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2158/1279340
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