The microscopic visualization of nanoparticles in plants is crucial to elucidate the mechanisms of their uptake through the cell wall and plasma membrane and to localize the possible sites of their extracellular or intracellular accumulation. Lignin nanocarriers are polymeric hollow nanocapsules able to contain and transport several bioactive substances inside plant tissues. We describe here a method for the preparation of Fluorol Yellow 088-labeled lignin nanocapsules that allow their localization in plant organs and tissues by fluorescence microscopy.

Fluorescent Labeling of Lignin Nanocapsules with Fluorol Yellow 088 / Cheli, Franco; Falsini, Sara; Salvatici, Maria Cristina; Ristori, Sandra; Schiff, Silvia; Corti, Emilio; Costantini, Irene; Gonnelli, Cristina; Pavone, Francesco Saverio; Papini, Alessio. - STAMPA. - (2023), pp. 345-353. [10.1007/978-1-0716-2675-7_28]

Fluorescent Labeling of Lignin Nanocapsules with Fluorol Yellow 088

Cheli, Franco;Falsini, Sara
;
Ristori, Sandra;Schiff, Silvia;Corti, Emilio;Costantini, Irene;Gonnelli, Cristina;Pavone, Francesco Saverio;Papini, Alessio
2023

Abstract

The microscopic visualization of nanoparticles in plants is crucial to elucidate the mechanisms of their uptake through the cell wall and plasma membrane and to localize the possible sites of their extracellular or intracellular accumulation. Lignin nanocarriers are polymeric hollow nanocapsules able to contain and transport several bioactive substances inside plant tissues. We describe here a method for the preparation of Fluorol Yellow 088-labeled lignin nanocapsules that allow their localization in plant organs and tissues by fluorescence microscopy.
2023
978-1-0716-2674-0
978-1-0716-2675-7
Histochemistry of Single Molecules. Methods and protocols.
345
353
Goal 3: Good health and well-being
Cheli, Franco; Falsini, Sara; Salvatici, Maria Cristina; Ristori, Sandra; Schiff, Silvia; Corti, Emilio; Costantini, Irene; Gonnelli, Cristina; Pavone...espandi
File in questo prodotto:
File Dimensione Formato  
161_Chelietal_Fluorescentlabelnanoparticles_28_Chapter.pdf

Accesso chiuso

Tipologia: Versione finale referata (Postprint, Accepted manuscript)
Licenza: Tutti i diritti riservati
Dimensione 354.39 kB
Formato Adobe PDF
354.39 kB Adobe PDF   Richiedi una copia

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1282840
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 1
  • ???jsp.display-item.citation.isi??? ND
social impact