The availability of more specific dyes for a subset of endomembrane compartments, combined with the development of genetically encoded probes and advanced microscopy technologies, makes live cell imaging an approach that goes beyond the microscopically observation of cell structure. Here we describe the latest improved techniques to investigate protein–protein interaction, protein topology, and protein dynamics. Furthermore, we depict new technical approaches to identify mutants for chloroplast morphology and distribution through the tracking of chlorophyll fluorescence, as well as mutants for chloroplast movement.
Live cell imaging and confocal microscopy / Giovanni Stefano; Luciana Renna. - ELETTRONICO. - (2018), pp. 117-130. [10.1007/978-1-4939-7856-4_9]
Live cell imaging and confocal microscopy
Giovanni StefanoWriting – Original Draft Preparation
;Luciana RennaWriting – Review & Editing
2018
Abstract
The availability of more specific dyes for a subset of endomembrane compartments, combined with the development of genetically encoded probes and advanced microscopy technologies, makes live cell imaging an approach that goes beyond the microscopically observation of cell structure. Here we describe the latest improved techniques to investigate protein–protein interaction, protein topology, and protein dynamics. Furthermore, we depict new technical approaches to identify mutants for chloroplast morphology and distribution through the tracking of chlorophyll fluorescence, as well as mutants for chloroplast movement.
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