Innovative Detection of the Quarantine Plant Pathogen Curtobacterium flaccumfaciens pv. flaccumfaciens, Causal Agent of Bacterial Wilt of Leguminous Plants

Specific, sensitive, and rapid detection of quarantine and regulated plant pathogens is pivotal for the control of the diseases they cause. Here, we describe the PCR-based methods which have been developed for Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff), quarantine plant pathogenic bacterium for EU and causal agent of bacterial wilt of Leguminous plants. These methods include an end-point and a real-time PCR test, and a LAMP assay. Their threshold analytical limits range from 100 to 10 fg of DNA template per reaction and are currently used worldwide for routine testing for Cff from laboratory to field scale.