As the world population is increasingly aging, it is important to identify protective interventions aimed to reduce the deleterious effects of aging, such as the increased incidence of diseases which are often disabling. Resveratrol is a stilbene compound produced by different plants, and red wine is the main source in the human diet. Resveratrol is endowed with many interesting biological activities, including an anti-aging effect which has been demonstrated both in vitro in eukariotic cells and in vivo. The aim of this work was to study the anti-aging activity of resveratrol in human fibroblasts in vitro, a widely used model in aging studies. We evaluated age-associated changes such as the increase in cellular and nuclear dimensions, and DNA content, DNA damage and replicative arrest. Global transcriptional changes were also evaluated by genome-wide gene expression microarray analysis, both during in vitro aging and after resveratrol treatment. We observed a biphasic effect of resveratrol, which at high concentrations inhibited cell proliferation, while at lower concentrations delayed the onset of replicative senescence. We also observed protective effects of resveratrol on DNA oxidative damage and resistance to in vitro induced oxidative stress, on nuclear DNA content and nuclear size in aging fibroblasts. Resveratrol treatment also had specific effects on the transcriptional profile of senescent fibroblasts, turning off complement and coagulation cascades and other innate immune response components typically activated during wound repair processes. In addition, resveratrol re-induced the basal expression of genes controlling cell cycle and DNA replication, usually down-regulated during aging. As a whole, these data are in agreement with an "anti-aging" activity of resveratrol.
Effects of resveratrol in aging: an in vitro study in human fibroblasts / V.Pitozzi; M.Jacomelli; A.Mocali; I.Cifola; C.Battaglia; L.Beltrame; N.Mulinacci; P.Dolara; L.Giovannelli. - ELETTRONICO. - (2009), pp. 169-169. (Intervento presentato al convegno SIF Società Italiana di Farmacologia tenutosi a Rimini nel 14-17 ottobre 2009).
Effects of resveratrol in aging: an in vitro study in human fibroblasts
PITOZZI, VANESSA;MOCALI, ALESSANDRA;MULINACCI, NADIA;DOLARA, PIERO;GIOVANNELLI, LISA
2009
Abstract
As the world population is increasingly aging, it is important to identify protective interventions aimed to reduce the deleterious effects of aging, such as the increased incidence of diseases which are often disabling. Resveratrol is a stilbene compound produced by different plants, and red wine is the main source in the human diet. Resveratrol is endowed with many interesting biological activities, including an anti-aging effect which has been demonstrated both in vitro in eukariotic cells and in vivo. The aim of this work was to study the anti-aging activity of resveratrol in human fibroblasts in vitro, a widely used model in aging studies. We evaluated age-associated changes such as the increase in cellular and nuclear dimensions, and DNA content, DNA damage and replicative arrest. Global transcriptional changes were also evaluated by genome-wide gene expression microarray analysis, both during in vitro aging and after resveratrol treatment. We observed a biphasic effect of resveratrol, which at high concentrations inhibited cell proliferation, while at lower concentrations delayed the onset of replicative senescence. We also observed protective effects of resveratrol on DNA oxidative damage and resistance to in vitro induced oxidative stress, on nuclear DNA content and nuclear size in aging fibroblasts. Resveratrol treatment also had specific effects on the transcriptional profile of senescent fibroblasts, turning off complement and coagulation cascades and other innate immune response components typically activated during wound repair processes. In addition, resveratrol re-induced the basal expression of genes controlling cell cycle and DNA replication, usually down-regulated during aging. As a whole, these data are in agreement with an "anti-aging" activity of resveratrol.I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.