Although human transthyretin (TTR) is associated with systemic amyloidoses, an anti-amyloidogenic effect that prevents Aβ fibril formation in vitro and in animalmodels has been observed. Herewe studied the ability of three different types of TTR, namely human tetramers (hTTR),mouse tetramers (muTTR) and an engineered monomer of the human protein (M-TTR), to suppress the toxicity of oligomers formed by two different amyloidogenic peptides/proteins (HypF-N and Aβ42). muTTR is the most stable homotetramer, hTTR can dissociate into partially unfolded monomers, whereas M-TTR maintains a monomeric state. Preformed toxic HypF-N and Aβ42 oligomers were incubated in the presence of each TTR then added to cell culture media. hTTR, and to a greater extent MTTR, were found to protect human neuroblastoma cells and rat primary neurons against oligomer-induced toxicity, whereas muTTR had no protective effect. The thioflavin T assay and site-directed labeling experiments using pyrene ruled out disaggregation and structural reorganizationwithin the discrete oligomers following incubation with TTRs, while confocal microscopy, SDS-PAGE, and intrinsic fluorescence measurements indicated tight binding between oligomers and hTTR, particularlyM-TTR.Moreover, atomic forcemicroscopy (AFM), light scattering and turbidimetry analyses indicated that larger assemblies of oligomers are formed in the presence ofM-TTR and, to a lesser extent, with hTTR. Overall, the data suggest a generic capacity of TTR to efficiently neutralize the toxicity of oligomers formed by misfolded proteins and reveal that such neutralization occurs through a mechanism of TTR-mediated assembly of protein oligomers into larger species, with an efficiency that correlates inversely with TTR tetramer stability.

Transthyretin suppresses the toxicity of oligomers formed by misfolded proteins in vitro / Roberta Cascella; Simona Conti; Benedetta Mannini; Xinyi Li; Joel N. Buxbaum; Bruno Tiribilli; Fabrizio Chiti; Cristina Cecchi. - In: BIOCHIMICA ET BIOPHYSICA ACTA. MOLECULAR BASIS OF DISEASE. - ISSN 0925-4439. - STAMPA. - 1832:(2013), pp. 2302-2314. [10.1016/j.bbadis.2013.09.011]

Transthyretin suppresses the toxicity of oligomers formed by misfolded proteins in vitro

CASCELLA, ROBERTA;CONTI, SIMONA;Benedetta Mannini;CHITI, FABRIZIO;CECCHI, CRISTINA
2013

Abstract

Although human transthyretin (TTR) is associated with systemic amyloidoses, an anti-amyloidogenic effect that prevents Aβ fibril formation in vitro and in animalmodels has been observed. Herewe studied the ability of three different types of TTR, namely human tetramers (hTTR),mouse tetramers (muTTR) and an engineered monomer of the human protein (M-TTR), to suppress the toxicity of oligomers formed by two different amyloidogenic peptides/proteins (HypF-N and Aβ42). muTTR is the most stable homotetramer, hTTR can dissociate into partially unfolded monomers, whereas M-TTR maintains a monomeric state. Preformed toxic HypF-N and Aβ42 oligomers were incubated in the presence of each TTR then added to cell culture media. hTTR, and to a greater extent MTTR, were found to protect human neuroblastoma cells and rat primary neurons against oligomer-induced toxicity, whereas muTTR had no protective effect. The thioflavin T assay and site-directed labeling experiments using pyrene ruled out disaggregation and structural reorganizationwithin the discrete oligomers following incubation with TTRs, while confocal microscopy, SDS-PAGE, and intrinsic fluorescence measurements indicated tight binding between oligomers and hTTR, particularlyM-TTR.Moreover, atomic forcemicroscopy (AFM), light scattering and turbidimetry analyses indicated that larger assemblies of oligomers are formed in the presence ofM-TTR and, to a lesser extent, with hTTR. Overall, the data suggest a generic capacity of TTR to efficiently neutralize the toxicity of oligomers formed by misfolded proteins and reveal that such neutralization occurs through a mechanism of TTR-mediated assembly of protein oligomers into larger species, with an efficiency that correlates inversely with TTR tetramer stability.
2013
1832
2302
2314
Roberta Cascella; Simona Conti; Benedetta Mannini; Xinyi Li; Joel N. Buxbaum; Bruno Tiribilli; Fabrizio Chiti; Cristina Cecchi
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/821884
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