Ruminants derived products have a prominent role in diets and economy worldwide; therefore, the capability to control the rumen microbial ecosystem, for ameliorating their quality, is of fundamental importance in the livestock sector. The aim of this study was to evaluate the effect of dietary supplementation with chestnut and quebracho tannins on microbial community and fatty acid profile, in the rumen fluid of dairy ewes. Multivariate analysis of PCR-DGGE profiles of rumen microbial communities showed a correlation among the presence of chestnut or quebracho in the diet, the specific Butyrivibrio group DGGE profiles, the increase in 18:3 cis9, cis12, and cis15; 18:2 cis9 and cis12; 18:2 cis9 and trans11; 18:2 trans11 and cis15; and 18:1 trans11 content, and the decrease in 18:0 concentration. Phylogenetic analysis of DGGE band sequences revealed the presence of bacteria representatives related to the genera Hungatella, Ruminococcus, and Eubacterium and unclassified Lachnospiraceae family members, suggesting that these taxa could be affected by tannins presence in the diets. The results of this study showed that tannins from chestnut and quebracho can reduce the biohydrogenation of unsaturated fatty acids through changes in rumen microbial communities.

Effect of Dietary Chestnut or Quebracho Tannin Supplementation on Microbial Community and Fatty Acid Profile in the Rumen of Dairy Ewes / Buccioni, Arianna; Pallara, Grazia; Pastorelli, Roberta; Bellini, Letizia; Cappucci, Alice; Mannelli, Federica; Minieri, Sara; Roscini, Valentina; Rapaccini, Stefano; Mele, Marcello; Giovannetti, Luciana; Viti, Carlo; Pauselli, Mariano. - In: BIOMED RESEARCH INTERNATIONAL. - ISSN 2314-6141. - ELETTRONICO. - 2017:(2017), pp. 1-12. [10.1155/2017/4969076]

Effect of Dietary Chestnut or Quebracho Tannin Supplementation on Microbial Community and Fatty Acid Profile in the Rumen of Dairy Ewes

Buccioni, Arianna
Investigation
;
Pallara, Grazia;Mannelli, Federica;Rapaccini, Stefano
Membro del Collaboration Group
;
Giovannetti, Luciana;Viti, Carlo
Investigation
;
2017

Abstract

Ruminants derived products have a prominent role in diets and economy worldwide; therefore, the capability to control the rumen microbial ecosystem, for ameliorating their quality, is of fundamental importance in the livestock sector. The aim of this study was to evaluate the effect of dietary supplementation with chestnut and quebracho tannins on microbial community and fatty acid profile, in the rumen fluid of dairy ewes. Multivariate analysis of PCR-DGGE profiles of rumen microbial communities showed a correlation among the presence of chestnut or quebracho in the diet, the specific Butyrivibrio group DGGE profiles, the increase in 18:3 cis9, cis12, and cis15; 18:2 cis9 and cis12; 18:2 cis9 and trans11; 18:2 trans11 and cis15; and 18:1 trans11 content, and the decrease in 18:0 concentration. Phylogenetic analysis of DGGE band sequences revealed the presence of bacteria representatives related to the genera Hungatella, Ruminococcus, and Eubacterium and unclassified Lachnospiraceae family members, suggesting that these taxa could be affected by tannins presence in the diets. The results of this study showed that tannins from chestnut and quebracho can reduce the biohydrogenation of unsaturated fatty acids through changes in rumen microbial communities.
2017
2017
1
12
Buccioni, Arianna; Pallara, Grazia; Pastorelli, Roberta; Bellini, Letizia; Cappucci, Alice; Mannelli, Federica; Minieri, Sara; Roscini, Valentina; Rapaccini, Stefano; Mele, Marcello; Giovannetti, Luciana; Viti, Carlo; Pauselli, Mariano
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Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/1110430
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