Surface Plasmon Resonance to Select and Characterize Recombinant Human Domain Antibodies.

Antibodies are a hallmark in Multiple Sclerosis (MS), and a group of them can be measured using CSF114(Glc), a synthetic peptide bearing an N-glucosyl group on Asn side chain, possibly because the peptide reproduces aberrant post-translational glycosylations on myelin proteins as target of autoantibodies [1]. It has already been demonstrated that in vitro panning procedures allow to obtain structure sensitive antibody fragments [2], and it is widely accepted that a single variable heavy (VH) domain isolated from natural antibodies may contribute to antigen binding with comparable affinities and specificities to whole antibody [3,4]. We reasoned that antibodies raised against this probe could have been relevant for MS. The aim of this work was the isolation of a panel of soluble hVH antibody fragments against the glucopeptide CSF114(Glc) from a synthetic human domain antibody library [5].