Evaluation of carotenoids, polyphenols and antioxidant activity in grain milled by stones

The functional quality of the semola, also related to the presence of secondary antioxidant metabolites, was evaluated by HPLC/DAD analysis and by in vitro spectrophotometric techniques. The total polyphenol content was evaluated with the Folin-Ciocalteau spectrophotometric method, and the evaluation of the carotenoid content was performed by HPLC / DAD chromatographic analysis and showed the presence of lutein as the main compound ranging between 75 and 78.5 % of total carotenoids. The chromatographic analyzes allowed us to identify the presence of an indolacetic acid derivative that varies between 0.097 and 0.108 mg/g.