Enzymatic degradation of pectin catalysed by Pectolyase Y23 RM (Sheishin Co., Japan) was studied in ultrafiltration membrane reactors. The mixtures of degradation products were continuously ultrafiltrated in four cells connected in series and equipped with membrane of decreasing molecular weight cut-off. The different fractions recovered. The ratio of enzyme to substrate loading affected product distribution and particularly the yield of oligomers from 10 to 15 monomeric units. TLC analysis allowed to identify the composition of the samples at low molecular weight (below 0.5 KDa and 1 KDa). The rate of pectin degradation in batch reactors was not significantly affected by the presence of oligomers between 1 and 6 subunits.
Pectin degradation in UF-membrane reactor with commercial pectinases / C. Dinnella; A. Stagni ; G. Lanzarini; F. Alfani; M. Cantarella; A. Gallifuoco. - STAMPA. - (1997), pp. 439-449. [10.1016/S0921-0423(96)80274-7]
Pectin degradation in UF-membrane reactor with commercial pectinases
DINNELLA, CATERINA;
1997
Abstract
Enzymatic degradation of pectin catalysed by Pectolyase Y23 RM (Sheishin Co., Japan) was studied in ultrafiltration membrane reactors. The mixtures of degradation products were continuously ultrafiltrated in four cells connected in series and equipped with membrane of decreasing molecular weight cut-off. The different fractions recovered. The ratio of enzyme to substrate loading affected product distribution and particularly the yield of oligomers from 10 to 15 monomeric units. TLC analysis allowed to identify the composition of the samples at low molecular weight (below 0.5 KDa and 1 KDa). The rate of pectin degradation in batch reactors was not significantly affected by the presence of oligomers between 1 and 6 subunits.
I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
