A commercial pectinase, immobilised on appropriately functionalised γ-alumina spheres, was loaded in a packed bed reactor and employed to depolymerise the pectin contained in a model solution and in the apple juice. The activity of the immobilized enzyme was tested in several batch reactions and compared with the one of the free enzyme. A successful apple juice depectinisation was obtained using the pectinase immobilised system. In addition, an endopolygalacturonase from Kluyveromyces marxianus, previously purified in a single-step process with coreshell microspheres specifically prepared, was immobilised on the same active support and the efficiency of the resulting catalyst was tested.
Immobilized pectinases behaviour and efficiency in the depolymerization of pectin in model solution and in apple juice / C. Dinnella; A. Stagni; G. Lanzarini. - STAMPA. - (1997), pp. 971-978. [10.1016/S0921-0423(96)80341-8]
Immobilized pectinases behaviour and efficiency in the depolymerization of pectin in model solution and in apple juice
DINNELLA, CATERINA;
1997
Abstract
A commercial pectinase, immobilised on appropriately functionalised γ-alumina spheres, was loaded in a packed bed reactor and employed to depolymerise the pectin contained in a model solution and in the apple juice. The activity of the immobilized enzyme was tested in several batch reactions and compared with the one of the free enzyme. A successful apple juice depectinisation was obtained using the pectinase immobilised system. In addition, an endopolygalacturonase from Kluyveromyces marxianus, previously purified in a single-step process with coreshell microspheres specifically prepared, was immobilised on the same active support and the efficiency of the resulting catalyst was tested.
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