HPLC Quantification of Flavonoids and Biflavonoids in Cupressaceae Leaves

The aim of this investigation was to obtain qualitative and quantitative profiles of the flavonoid and biflavonoid composition of six cypress species - Cupressus funebris L., Cupressus semper- Wrens L., Cupressus glabra L., Cupressus arizonica L., Cupressus goveniana L., and Cupressus lusitanica L. HPLC-diode-array detection (DAD), HPLC-MS, and HPTLC were used to identify the individual compounds. A chromatographic method was optimized for identification and quantification of the main flavonoid glycosides and biflavonoids. The flavonoids identified and calibrated were: rutin, quercetin glucoside, quercetin rhamnoside, and kaempferol 3-0- rhamnoside. The biflavonoids identified and calibrated were: cu pressuflavone, amentoflavone, robustaflavone, hinokiflavone, methylrobustaflavone, methylamentoflavone, and dimethylcupressuflavone.