The aim of this investigation was to obtain qualitative and quantitative profiles of the flavonoid and biflavonoid composition of six cypress species - Cupressus funebris L., Cupressus semper- Wrens L., Cupressus glabra L., Cupressus arizonica L., Cupressus goveniana L., and Cupressus lusitanica L. HPLC-diode-array detection (DAD), HPLC-MS, and HPTLC were used to identify the individual compounds. A chromatographic method was optimized for identification and quantification of the main flavonoid glycosides and biflavonoids. The flavonoids identified and calibrated were: rutin, quercetin glucoside, quercetin rhamnoside, and kaempferol 3-0- rhamnoside. The biflavonoids identified and calibrated were: cu pressuflavone, amentoflavone, robustaflavone, hinokiflavone, methylrobustaflavone, methylamentoflavone, and dimethylcupressuflavone.
HPLC Quantification of Flavonoids and Biflavonoids in Cupressaceae Leaves / A. ROMANI; C.GALARDI; P.PINELLI; N.MULINACCI; D.HEIMLER. - In: CHROMATOGRAPHIA. - ISSN 0009-5893. - ELETTRONICO. - 56 (7/8):(2002), pp. 469-474. [10.1007/BF02492011]
HPLC Quantification of Flavonoids and Biflavonoids in Cupressaceae Leaves
ROMANI, ANNALISA;PINELLI, PATRIZIA;MULINACCI, NADIA;HEIMLER, DANIELA
2002
Abstract
The aim of this investigation was to obtain qualitative and quantitative profiles of the flavonoid and biflavonoid composition of six cypress species - Cupressus funebris L., Cupressus semper- Wrens L., Cupressus glabra L., Cupressus arizonica L., Cupressus goveniana L., and Cupressus lusitanica L. HPLC-diode-array detection (DAD), HPLC-MS, and HPTLC were used to identify the individual compounds. A chromatographic method was optimized for identification and quantification of the main flavonoid glycosides and biflavonoids. The flavonoids identified and calibrated were: rutin, quercetin glucoside, quercetin rhamnoside, and kaempferol 3-0- rhamnoside. The biflavonoids identified and calibrated were: cu pressuflavone, amentoflavone, robustaflavone, hinokiflavone, methylrobustaflavone, methylamentoflavone, and dimethylcupressuflavone.File | Dimensione | Formato | |
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