A new series of pyrazolo[1',5':1,6]pyrimido[4,5-d]pyridazin-4(3H)-ones was synthesized and tested in radioligand binding assays on human A1, A2A and A3 adenosine receptors. Most of the compounds showed high selectivity of action towards A1 receptor and high affinity with Ki values in the low nanomolar range. The pharmacological profile of the most active molecules towards A1 adenosine receptors was evaluated in cAMP functional assay. Compounds demonstrated their ability to completely counteract the effect of the agonist NECA, thus demonstrating their antagonist profile. Moreover, the most interesting compound, tested in the mouse passive avoidance, exhibited an antiamnesic effect at the doses of 10 and 30 mg/kg.

Further studies on pyrazolo[1',5':1,6]pyrimido[4,5-d]pyridazin-4(3H)-ones as potent and selective human A1 adenosine receptor antagonists / M. P. Giovannoni;G. Ciciani;A. Cilibrizzi;L. Crocetti;S. Daniele;L. Di Cesare Mannelli;C. Ghelardini;C. Giacomelli;G. Guerrini;C. Martini;M. L. Trincavelli;C. Vergelli. - In: EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY. - ISSN 0223-5234. - ELETTRONICO. - 89:(2015), pp. 32-41. [10.1016/j.ejmech.2014.10.020]

Further studies on pyrazolo[1',5':1,6]pyrimido[4,5-d]pyridazin-4(3H)-ones as potent and selective human A1 adenosine receptor antagonists.

GIOVANNONI, MARIA PAOLA;CICIANI, GIOVANNA;CROCETTI, LETIZIA;DI CESARE MANNELLI, LORENZO;GHELARDINI, CARLA;GUERRINI, GABRIELLA;VERGELLI, CLAUDIA
2015

Abstract

A new series of pyrazolo[1',5':1,6]pyrimido[4,5-d]pyridazin-4(3H)-ones was synthesized and tested in radioligand binding assays on human A1, A2A and A3 adenosine receptors. Most of the compounds showed high selectivity of action towards A1 receptor and high affinity with Ki values in the low nanomolar range. The pharmacological profile of the most active molecules towards A1 adenosine receptors was evaluated in cAMP functional assay. Compounds demonstrated their ability to completely counteract the effect of the agonist NECA, thus demonstrating their antagonist profile. Moreover, the most interesting compound, tested in the mouse passive avoidance, exhibited an antiamnesic effect at the doses of 10 and 30 mg/kg.
2015
89
32
41
Goal 3: Good health and well-being for people
M. P. Giovannoni;G. Ciciani;A. Cilibrizzi;L. Crocetti;S. Daniele;L. Di Cesare Mannelli;C. Ghelardini;C. Giacomelli;G. Guerrini;C. Martini;M....espandi
File in questo prodotto:
File Dimensione Formato  
2015 EJMC adenosina A1 paola.pdf

Accesso chiuso

Tipologia: Pdf editoriale (Version of record)
Licenza: Tutti i diritti riservati
Dimensione 669.51 kB
Formato Adobe PDF
669.51 kB Adobe PDF   Richiedi una copia
EJMECH-S-14-01449.pdf

accesso aperto

Tipologia: Altro
Licenza: Creative commons
Dimensione 740.92 kB
Formato Adobe PDF
740.92 kB Adobe PDF

I documenti in FLORE sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificatore per citare o creare un link a questa risorsa: https://hdl.handle.net/2158/960403
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 17
  • ???jsp.display-item.citation.isi??? 17
social impact